If we are able to connect an alien DNA piece with plasmid DNA or a bacteriophage, we are able to multiply its number equal to the copy number of plasmids and bacteriophage respectively. “Bioreactors are large vessels in which raw materials are biologically converted into specific products by microbes, plants and animals.” The reactors work by providing optimum condition to the process of culture by maintaining optimum temperature, oxygen, salt, pH and other required conditions. This idea plays the significant role in genetic engineering and has resulted in the creation of recombinant DNA, gene transfer and gene cloning. The principle of genetic engineering is to manipulate and modify the genetic material of an organism or plants to insert desirable traits. This specific sequence of base pair is referred as recognition sequence. The following sequence reads the same on two strands in 5’ → 3’ direction. For instance, one of the biotechnological technique is the application of fermentation in alcohol production. Explain the contribution of Thermus aquaticus in the amplification of a gene of interest. Complete List of Packages for Medical Preparation, Conceptual development of concepts of Genetic Engineering, Separation and Isolation of DNA fragments, Competent Host (For Transformation with Recombinant DNA). Added to this, plasmids have only 1 - 2 copies per cell. Genetic Engineering – It includes several techniques that facilitate the alteration of genetic material, that is, RNA or DNA, in order to introduce them in host organisms. Genetic Engineering. The restriction enzymes belong to larger class of enzymes called nucleases which are further classified as endonucleases and exonucleases. Biotechnology is the use of technology for modifying or manipulating a biological system. Restriction endonucleases form DNA fragments that are separated via technique gel electrophoresis. 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